4,4'-Dimethoxytrityl chlorideiyinhlangano enamandla ye-nucleoside ne-nucleotide evikela futhi eqeda i-ejenti.
Igama elifanayo:I-DMT-Cl
Inombolo ye-CAS:40615-36-9
Izakhiwo
I-Chemical Formula
Isisindo samangqamuzana
Izinga Lokushisa Lokugcina
I-Melting Point
Ubumsulwa
Ingaphandle
I-C21H19ClO2
338.82 g/mol
2 ~ 8℃
120-125 ℃
≥98%
I-Pink Crystalline
Njengezingxenye ezibalulekile zamaketanga e-nucleic acid, ama-nucleoside nama-nucleotide adlala indima ebalulekile ekudlulisweni kolwazi lofuzo.Kodwa-ke, ngenxa ye-reactivity yabo yamakhemikhali kanye nokuwohloka kalula, ama-nucleoside nama-nucleotide adinga ama-reagents akhethekile ukuze awavikele ngesikhathi sokuhlanganiswa.I-DMTCl4' Dimethoxytrityl (DMTCl) iyiqembu elisebenza ngempumelelo kakhulu elivikela futhi likhiphe i-ejenti eguqule ukuvikelwa kwama-nucleoside nama-nucleotide ngesikhathi sokuhlanganiswa.
I-DMTCl iyi-ejenti evikela i-hydroxyl esabela ngokukhetha kanye namaqembu e-hydroxyl e-nucleosides nama-nucleotide ngesikhathi sokuhlanganiswa ukuze yakhe iqembu elivikelayo lesikhashana elivimbela ama-nucleoside nama-nucleotide ukuthi angasabelani namanye amakhemikhali.Leli qembu elivikelayo lesikhashana lisuswa kalula ngemva kokuhlanganiswa, lishiya ama-nucleoside ahlanzekile noma ama-nucleotide.
Ngenxa ye-reactivity yayo enhle kakhulu nokukhetha,I-DMTClisetshenziswa kabanzi njengeqembu elivikela futhi liqede i-ejenti ekuhlanganiseni kwe-DNA, i-RNA namanye ama-nucleic acid.Kuwusizo ikakhulukazi ku-oligonucleotide synthesis lapho ukulawulwa okunembile phezu kwendawo ye-nucleotide nokulandelana kuyadingeka.
Ngaphezu kokusebenza njengomvikeli weqembu nesinqamuli, i-DMTCl iwumvikeli onamandla we-hydroxyl wama-nucleoside nama-nucleotide.Ivikela amaqembu e-hydroxyl we-nucleosides nama-nucleotide ekufakweni kwe-oxidation, ekwehliseni, nokunye ukusabela okungawalimaza ngesikhathi sokuhlanganiswa.
Ngokufigqiwe,I-DMTCl4 4' dimethoxytrityliyithuluzi elibalulekile lokuhlanganiswa kwama-nucleoside nama-nucleotide.Ukusebenza kabusha kwayo okuhle kakhulu, ukukhetha nokuguquguquka kuyenza iqembu elikahle elivikela futhi liqede i-ejenti kanye ne-ejenti evikela i-hydroxyl ye-DNA, i-RNA nokunye ukuhlanganiswa kwe-nucleic acid.Uma udinga ukuvikelwa kwekhwalithi ephezulu nokuthembekile kwama-nucleosides nama-nucleotide ngesikhathi sokuhlanganiswa, ungabheki kude kune-DMTCl.
Isikhathi sokuthumela: Jun-08-2023